UPDATE | June 9, 2026
High-yield AAV production using a “mini-helper” plasmid with optimized strong promoters
Improving AAV production efficiency requires helper plasmid strategies that balance DNA input, vector yield, and scalability.
This poster presents Samsung Biologics’ Mini-Helper plasmid approach, which retains only essential helper elements while optimizing promoter design to improve AAV production across multiple serotypes.
Key highlights include:
Minimized helper plasmid design retaining only essential elements
Reduced DNA input while maintaining or improving AAV titer
Improved AAV titer and packaging efficiency compared with conventional helper plasmids
Consistent AAV yield improvements across flask and 2 L bioreactor scales
Improved VG titer and cost-efficiency supporting scalable AAV production
Discover how Samsung Biologics’ Mini-Helper approach can support more efficient AAV vector production while reducing DNA requirements and maintaining scalability.
Improving AAV production efficiency requires helper plasmid strategies that balance DNA input, vector yield, and scalability.
This poster presents Samsung Biologics’ Mini-Helper plasmid approach, which retains only essential helper elements while optimizing promoter design to improve AAV production across multiple serotypes.
Key highlights include:
- Minimized helper plasmid design retaining only essential elements
- Reduced DNA input while maintaining or improving AAV titer
- Improved AAV titer and packaging efficiency compared with conventional helper plasmids
- Consistent AAV yield improvements across flask and 2 L bioreactor scales
- Improved VG titer and cost-efficiency supporting scalable AAV production
Discover how Samsung Biologics’ Mini-Helper approach can support more efficient AAV vector production while reducing DNA requirements and maintaining scalability.
Related Content