UPDATE | June 9, 2026
High-yield AAV production using a “mini-helper” plasmid with optimized strong promoters
Conventional helper plasmids can contain large non-essential regions that increase DNA synthesis costs and reduce transfection efficiency. A minimized “Mini-Helper” plasmid was engineered to address these limitations by retaining only essential elements and incorporating optimized strong promoters.
This poster presents Samsung Biologics’ Mini-Helper plasmid approach, demonstrating how plasmid minimization and promoter optimization can improve AAV production across multiple serotypes.
Key highlights include:
Minimized helper plasmid design retaining only essential elements
Reduced DNA input while increasing AAV titer
Improved AAV titer and packaging efficiency compared with conventional helper plasmids
Consistent AAV yield improvements across flask and 2 L bioreactor scales
Improved VG titer and cost-efficiency for scalable AAV production
Discover how Samsung Biologics’ Mini-Helper approach can support more efficient and scalable AAV vector production through plasmid minimization and promoter optimization.
Conventional helper plasmids can contain large non-essential regions that increase DNA synthesis costs and reduce transfection efficiency. A minimized “Mini-Helper” plasmid was engineered to address these limitations by retaining only essential elements and incorporating optimized strong promoters.
This poster presents Samsung Biologics’ Mini-Helper plasmid approach, demonstrating how plasmid minimization and promoter optimization can improve AAV production across multiple serotypes.
Key highlights include:
- Minimized helper plasmid design retaining only essential elements
- Reduced DNA input while increasing AAV titer
- Improved AAV titer and packaging efficiency compared with conventional helper plasmids
- Consistent AAV yield improvements across flask and 2 L bioreactor scales
- Improved VG titer and cost-efficiency for scalable AAV production
Discover how Samsung Biologics’ Mini-Helper approach can support more efficient and scalable AAV vector production through plasmid minimization and promoter optimization.
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